Journal: International Journal of Molecular Sciences

Article Title: Leptin Silencing Attenuates Lipid Accumulation through Sterol Regulatory Element-Binding Protein 1 Inhibition in Nasopharyngeal Carcinoma

doi: 10.3390/ijms23105700

Figure Lengend Snippet: Leptin is involved in lipid metabolism in NPC cells. ( A , B ) The SREBP1 expression level was estimated by QPCR and Western blotting after leptin was silenced in TW02 and TW06 cells. ( C ) The luciferase assay was performed to determine the transcriptional activity of SREBP1 in leptin-depleted NPC cells and negative control cells. ( D ) The FASN and SCD1 at mRNA expression levels were estimated by QPCR after leptin was silenced in TW02 and TW06 cells. All data were obtained from three independent experiments. Data are presented as the mean ± SD. *: p < 0.05; **: p < 0.01; ***: p < 0.001; n.s.: not significant.

Article Snippet: The PCR-amplified human full-length SREBP1 cDNA was constructed into the mammalian cell expression vector pEntry (OriGene Technologies, Rockville, MD, USA).

Techniques: Expressing, Western Blot, Luciferase, Activity Assay, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Leptin Silencing Attenuates Lipid Accumulation through Sterol Regulatory Element-Binding Protein 1 Inhibition in Nasopharyngeal Carcinoma

doi: 10.3390/ijms23105700

Figure Lengend Snippet: Leptin deficiency decreases SREBP1 via upregulation of PPAR-γ in NPC cells. ( A ) Western blot analysis of PPAR-γ and SREBP1 in leptin-depleted NPC cells in the absence or presence of GW9662. ( B , C ) The levels of TG and cholesterol in the indicated NPC cells with GW9662 treatment were measured. All data were obtained from three independent experiments. Data are presented as the mean ± SD. *: p < 0.05; **: p < 0.01; ***: p < 0.001.

Article Snippet: The PCR-amplified human full-length SREBP1 cDNA was constructed into the mammalian cell expression vector pEntry (OriGene Technologies, Rockville, MD, USA).

Techniques: Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Leptin Silencing Attenuates Lipid Accumulation through Sterol Regulatory Element-Binding Protein 1 Inhibition in Nasopharyngeal Carcinoma

doi: 10.3390/ijms23105700

Figure Lengend Snippet: SREBP1 expression contributes to leptin-elicited NPC cell proliferation and lipogenesis. ( A ) Western blotting analysis of SREBP1 expression levels in shleptin-TW02 and shleptin-TW06 cells transfected with a vector alone or SREBP1 plasmid. ( B ) The CCK8 and colony formation assays determined the cell growth ability of leptin-silenced TW02 with SREBP1 expression or vector alone. ( C ) The levels of intracellular TG and cholesterol in shleptin-TW02 and shleptin-TW06 cells with SREBP1 expression were determined. v: vector; NC: shcontrol; Flag-S: Flag-SREBP1. All data were obtained from three independent experiments. Data are presented as the mean ± SD. *: p < 0.05; **: p < 0.01; ***: p < 0.001.

Article Snippet: The PCR-amplified human full-length SREBP1 cDNA was constructed into the mammalian cell expression vector pEntry (OriGene Technologies, Rockville, MD, USA).

Techniques: Expressing, Western Blot, Transfection, Plasmid Preparation

Journal: International Journal of Molecular Sciences

Article Title: Leptin Silencing Attenuates Lipid Accumulation through Sterol Regulatory Element-Binding Protein 1 Inhibition in Nasopharyngeal Carcinoma

doi: 10.3390/ijms23105700

Figure Lengend Snippet: Leptin inhibition attenuates SREBP1 expression in vivo. ( A ) Mouse body weights were measured in shcontrol and shleptin groups. ( B ) IHC was performed to investigate the protein expression levels of SREBP1, FASN, and SCD1 in shcontrol and shleptin xenograft tumors. The quantifications of IHC staining are shown. The data are presented as the mean ± SD. ***: p < 0.001; n.s.: not significant.

Article Snippet: The PCR-amplified human full-length SREBP1 cDNA was constructed into the mammalian cell expression vector pEntry (OriGene Technologies, Rockville, MD, USA).

Techniques: Inhibition, Expressing, In Vivo, Immunohistochemistry

Journal: iScience

Article Title: Dominant-negative HNF1α mutant promotes liver steatosis and inflammation by regulating hepatic complement factor D

doi: 10.1016/j.isci.2023.108018

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-SREBP-1C (Western blot 1:1000) , Novus, USA , Cat#NB600-582; RRID: AB_10001575.

Techniques: Western Blot, Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Reporter Assay, Transfection, Mutagenesis, cDNA Synthesis, SYBR Green Assay, Plasmid Preparation, Software

Journal: BMC Medical Genetics

Article Title: Effects of interacting networks of cardiovascular risk genes on the risk of type 2 diabetes mellitus (the CODAM study)

doi: 10.1186/1471-2350-9-36

Figure Lengend Snippet: Polymorphisms used in genotyping assay.

Article Snippet: Interon 14 G+1A (V58) , 16q21 , CETP , 0.

Techniques: